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immunosorbent assay elisa max standard kit  (Revvity)


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    Revvity immunosorbent assay elisa max standard kit
    Under P. gingivalis stimulation for 24 h, the CD45 + IL-10 + cells, IL-10 mRNA level and secreted IL-10 protein levels of splenocytes and B cells isolated from pre-immunized TLR9 knockout mice were calculated. ( A ) The representative ICC images of CD45 + IL-10 + cells in each group. CD45 cells were labeled with rat anti-mouse CD45 antibody conjugated with emerald anti-rat antibody (blue) and IL-10 were labeled with goat anti-mouse IL-10 antibody conjugated with permanent red anti-goat antibody (red). ( B , E ) The percentage of CD45 + IL-10 + cells among splenocytes ( B ) and B cells ( E ) were calculated based on cell immunocytochemistry staining. ( C , F ) The IL-10 mRNA expression in splenocytes ( C ) and B cells ( F ) was analyzed by quantitative RT-PCR. ( D , G ) The secreted IL-10 protein levels in the supernatants were measured by a mouse IL-10 enzyme-linked <t>ELISA</t> kit using a 1:10 assay diluent in PBST. The data were shown as mean ± SD; significance calculated by unpaired t -test was indicated as ** p < 0.01, **** p < 0.0001, and ns (no significant difference) as p > 0.05 (n = 5).
    Immunosorbent Assay Elisa Max Standard Kit, supplied by Revvity, used in various techniques. Bioz Stars score: 92/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunosorbent assay elisa max standard kit/product/Revvity
    Average 92 stars, based on 119 article reviews
    immunosorbent assay elisa max standard kit - by Bioz Stars, 2026-02
    92/100 stars

    Images

    1) Product Images from "TLR9 Signaling Is Required for the Porphyromonas gingivalis -Induced Activation of IL-10-Expressing B Cells"

    Article Title: TLR9 Signaling Is Required for the Porphyromonas gingivalis -Induced Activation of IL-10-Expressing B Cells

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms24076693

    Under P. gingivalis stimulation for 24 h, the CD45 + IL-10 + cells, IL-10 mRNA level and secreted IL-10 protein levels of splenocytes and B cells isolated from pre-immunized TLR9 knockout mice were calculated. ( A ) The representative ICC images of CD45 + IL-10 + cells in each group. CD45 cells were labeled with rat anti-mouse CD45 antibody conjugated with emerald anti-rat antibody (blue) and IL-10 were labeled with goat anti-mouse IL-10 antibody conjugated with permanent red anti-goat antibody (red). ( B , E ) The percentage of CD45 + IL-10 + cells among splenocytes ( B ) and B cells ( E ) were calculated based on cell immunocytochemistry staining. ( C , F ) The IL-10 mRNA expression in splenocytes ( C ) and B cells ( F ) was analyzed by quantitative RT-PCR. ( D , G ) The secreted IL-10 protein levels in the supernatants were measured by a mouse IL-10 enzyme-linked ELISA kit using a 1:10 assay diluent in PBST. The data were shown as mean ± SD; significance calculated by unpaired t -test was indicated as ** p < 0.01, **** p < 0.0001, and ns (no significant difference) as p > 0.05 (n = 5).
    Figure Legend Snippet: Under P. gingivalis stimulation for 24 h, the CD45 + IL-10 + cells, IL-10 mRNA level and secreted IL-10 protein levels of splenocytes and B cells isolated from pre-immunized TLR9 knockout mice were calculated. ( A ) The representative ICC images of CD45 + IL-10 + cells in each group. CD45 cells were labeled with rat anti-mouse CD45 antibody conjugated with emerald anti-rat antibody (blue) and IL-10 were labeled with goat anti-mouse IL-10 antibody conjugated with permanent red anti-goat antibody (red). ( B , E ) The percentage of CD45 + IL-10 + cells among splenocytes ( B ) and B cells ( E ) were calculated based on cell immunocytochemistry staining. ( C , F ) The IL-10 mRNA expression in splenocytes ( C ) and B cells ( F ) was analyzed by quantitative RT-PCR. ( D , G ) The secreted IL-10 protein levels in the supernatants were measured by a mouse IL-10 enzyme-linked ELISA kit using a 1:10 assay diluent in PBST. The data were shown as mean ± SD; significance calculated by unpaired t -test was indicated as ** p < 0.01, **** p < 0.0001, and ns (no significant difference) as p > 0.05 (n = 5).

    Techniques Used: Isolation, Knock-Out, Labeling, Immunocytochemistry, Staining, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

    Under P. gingivalis stimulation for 24 h, the CD45 + IL-10 + cells of splenocytes and B cells, and secreted IL-10 protein levels of B cells isolated from both pre-immunized wild-type and TLR9 knockout mice were calculated. ( A , B ) The percentage of CD45 + IL-10 + cells among splenocytes ( A ) and B cells ( B ) were calculated based on cell immunocytochemistry staining. ( C ) The secreted IL-10 protein levels in the supernatants were measured by a mouse IL-10 enzyme-linked ELISA kit using a 1:10 assay diluent in PBST. The data were shown as mean ± SD; significance calculated by unpaired t -test was indicated as * p < 0.05, **** p < 0.0001 (n = 5).
    Figure Legend Snippet: Under P. gingivalis stimulation for 24 h, the CD45 + IL-10 + cells of splenocytes and B cells, and secreted IL-10 protein levels of B cells isolated from both pre-immunized wild-type and TLR9 knockout mice were calculated. ( A , B ) The percentage of CD45 + IL-10 + cells among splenocytes ( A ) and B cells ( B ) were calculated based on cell immunocytochemistry staining. ( C ) The secreted IL-10 protein levels in the supernatants were measured by a mouse IL-10 enzyme-linked ELISA kit using a 1:10 assay diluent in PBST. The data were shown as mean ± SD; significance calculated by unpaired t -test was indicated as * p < 0.05, **** p < 0.0001 (n = 5).

    Techniques Used: Isolation, Knock-Out, Immunocytochemistry, Staining, Enzyme-linked Immunosorbent Assay



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    Revvity immunosorbent assay elisa max standard kit
    Under P. gingivalis stimulation for 24 h, the CD45 + IL-10 + cells, IL-10 mRNA level and secreted IL-10 protein levels of splenocytes and B cells isolated from pre-immunized TLR9 knockout mice were calculated. ( A ) The representative ICC images of CD45 + IL-10 + cells in each group. CD45 cells were labeled with rat anti-mouse CD45 antibody conjugated with emerald anti-rat antibody (blue) and IL-10 were labeled with goat anti-mouse IL-10 antibody conjugated with permanent red anti-goat antibody (red). ( B , E ) The percentage of CD45 + IL-10 + cells among splenocytes ( B ) and B cells ( E ) were calculated based on cell immunocytochemistry staining. ( C , F ) The IL-10 mRNA expression in splenocytes ( C ) and B cells ( F ) was analyzed by quantitative RT-PCR. ( D , G ) The secreted IL-10 protein levels in the supernatants were measured by a mouse IL-10 enzyme-linked <t>ELISA</t> kit using a 1:10 assay diluent in PBST. The data were shown as mean ± SD; significance calculated by unpaired t -test was indicated as ** p < 0.01, **** p < 0.0001, and ns (no significant difference) as p > 0.05 (n = 5).
    Immunosorbent Assay Elisa Max Standard Kit, supplied by Revvity, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunosorbent assay elisa max standard kit/product/Revvity
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    immunosorbent assay elisa max kit  (Revvity)
    92
    Revvity immunosorbent assay elisa max kit
    Under P. gingivalis stimulation for 24 h, the CD45 + IL-10 + cells, IL-10 mRNA level and secreted IL-10 protein levels of splenocytes and B cells isolated from pre-immunized TLR9 knockout mice were calculated. ( A ) The representative ICC images of CD45 + IL-10 + cells in each group. CD45 cells were labeled with rat anti-mouse CD45 antibody conjugated with emerald anti-rat antibody (blue) and IL-10 were labeled with goat anti-mouse IL-10 antibody conjugated with permanent red anti-goat antibody (red). ( B , E ) The percentage of CD45 + IL-10 + cells among splenocytes ( B ) and B cells ( E ) were calculated based on cell immunocytochemistry staining. ( C , F ) The IL-10 mRNA expression in splenocytes ( C ) and B cells ( F ) was analyzed by quantitative RT-PCR. ( D , G ) The secreted IL-10 protein levels in the supernatants were measured by a mouse IL-10 enzyme-linked <t>ELISA</t> kit using a 1:10 assay diluent in PBST. The data were shown as mean ± SD; significance calculated by unpaired t -test was indicated as ** p < 0.01, **** p < 0.0001, and ns (no significant difference) as p > 0.05 (n = 5).
    Immunosorbent Assay Elisa Max Kit, supplied by Revvity, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunosorbent assay elisa max kit/product/Revvity
    Average 92 stars, based on 1 article reviews
    immunosorbent assay elisa max kit - by Bioz Stars, 2026-02
    92/100 stars
    		  Buy from Supplier
    immunosorbent assay elisa max kits  (Revvity)
    92
    Revvity immunosorbent assay elisa max kits
    Under P. gingivalis stimulation for 24 h, the CD45 + IL-10 + cells, IL-10 mRNA level and secreted IL-10 protein levels of splenocytes and B cells isolated from pre-immunized TLR9 knockout mice were calculated. ( A ) The representative ICC images of CD45 + IL-10 + cells in each group. CD45 cells were labeled with rat anti-mouse CD45 antibody conjugated with emerald anti-rat antibody (blue) and IL-10 were labeled with goat anti-mouse IL-10 antibody conjugated with permanent red anti-goat antibody (red). ( B , E ) The percentage of CD45 + IL-10 + cells among splenocytes ( B ) and B cells ( E ) were calculated based on cell immunocytochemistry staining. ( C , F ) The IL-10 mRNA expression in splenocytes ( C ) and B cells ( F ) was analyzed by quantitative RT-PCR. ( D , G ) The secreted IL-10 protein levels in the supernatants were measured by a mouse IL-10 enzyme-linked <t>ELISA</t> kit using a 1:10 assay diluent in PBST. The data were shown as mean ± SD; significance calculated by unpaired t -test was indicated as ** p < 0.01, **** p < 0.0001, and ns (no significant difference) as p > 0.05 (n = 5).
    Immunosorbent Assay Elisa Max Kits, supplied by Revvity, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunosorbent assay elisa max kits/product/Revvity
    Average 92 stars, based on 1 article reviews
    immunosorbent assay elisa max kits - by Bioz Stars, 2026-02
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Under P. gingivalis stimulation for 24 h, the CD45 + IL-10 + cells, IL-10 mRNA level and secreted IL-10 protein levels of splenocytes and B cells isolated from pre-immunized TLR9 knockout mice were calculated. ( A ) The representative ICC images of CD45 + IL-10 + cells in each group. CD45 cells were labeled with rat anti-mouse CD45 antibody conjugated with emerald anti-rat antibody (blue) and IL-10 were labeled with goat anti-mouse IL-10 antibody conjugated with permanent red anti-goat antibody (red). ( B , E ) The percentage of CD45 + IL-10 + cells among splenocytes ( B ) and B cells ( E ) were calculated based on cell immunocytochemistry staining. ( C , F ) The IL-10 mRNA expression in splenocytes ( C ) and B cells ( F ) was analyzed by quantitative RT-PCR. ( D , G ) The secreted IL-10 protein levels in the supernatants were measured by a mouse IL-10 enzyme-linked ELISA kit using a 1:10 assay diluent in PBST. The data were shown as mean ± SD; significance calculated by unpaired t -test was indicated as ** p < 0.01, **** p < 0.0001, and ns (no significant difference) as p > 0.05 (n = 5).

    Journal: International Journal of Molecular Sciences

    Article Title: TLR9 Signaling Is Required for the Porphyromonas gingivalis -Induced Activation of IL-10-Expressing B Cells

    doi: 10.3390/ijms24076693

    Figure Lengend Snippet: Under P. gingivalis stimulation for 24 h, the CD45 + IL-10 + cells, IL-10 mRNA level and secreted IL-10 protein levels of splenocytes and B cells isolated from pre-immunized TLR9 knockout mice were calculated. ( A ) The representative ICC images of CD45 + IL-10 + cells in each group. CD45 cells were labeled with rat anti-mouse CD45 antibody conjugated with emerald anti-rat antibody (blue) and IL-10 were labeled with goat anti-mouse IL-10 antibody conjugated with permanent red anti-goat antibody (red). ( B , E ) The percentage of CD45 + IL-10 + cells among splenocytes ( B ) and B cells ( E ) were calculated based on cell immunocytochemistry staining. ( C , F ) The IL-10 mRNA expression in splenocytes ( C ) and B cells ( F ) was analyzed by quantitative RT-PCR. ( D , G ) The secreted IL-10 protein levels in the supernatants were measured by a mouse IL-10 enzyme-linked ELISA kit using a 1:10 assay diluent in PBST. The data were shown as mean ± SD; significance calculated by unpaired t -test was indicated as ** p < 0.01, **** p < 0.0001, and ns (no significant difference) as p > 0.05 (n = 5).

    Article Snippet: The secreted IL-10 protein levels in the supernatants of cultured splenocytes and B cells were measured by a mouse IL-10 enzyme-linked immunosorbent assay (ELISA) Max Standard kit (BioLegend, San Diego, CA, USA) using 1:10 assay diluent in PBST.

    Techniques: Isolation, Knock-Out, Labeling, Immunocytochemistry, Staining, Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

    Under P. gingivalis stimulation for 24 h, the CD45 + IL-10 + cells of splenocytes and B cells, and secreted IL-10 protein levels of B cells isolated from both pre-immunized wild-type and TLR9 knockout mice were calculated. ( A , B ) The percentage of CD45 + IL-10 + cells among splenocytes ( A ) and B cells ( B ) were calculated based on cell immunocytochemistry staining. ( C ) The secreted IL-10 protein levels in the supernatants were measured by a mouse IL-10 enzyme-linked ELISA kit using a 1:10 assay diluent in PBST. The data were shown as mean ± SD; significance calculated by unpaired t -test was indicated as * p < 0.05, **** p < 0.0001 (n = 5).

    Journal: International Journal of Molecular Sciences

    Article Title: TLR9 Signaling Is Required for the Porphyromonas gingivalis -Induced Activation of IL-10-Expressing B Cells

    doi: 10.3390/ijms24076693

    Figure Lengend Snippet: Under P. gingivalis stimulation for 24 h, the CD45 + IL-10 + cells of splenocytes and B cells, and secreted IL-10 protein levels of B cells isolated from both pre-immunized wild-type and TLR9 knockout mice were calculated. ( A , B ) The percentage of CD45 + IL-10 + cells among splenocytes ( A ) and B cells ( B ) were calculated based on cell immunocytochemistry staining. ( C ) The secreted IL-10 protein levels in the supernatants were measured by a mouse IL-10 enzyme-linked ELISA kit using a 1:10 assay diluent in PBST. The data were shown as mean ± SD; significance calculated by unpaired t -test was indicated as * p < 0.05, **** p < 0.0001 (n = 5).

    Article Snippet: The secreted IL-10 protein levels in the supernatants of cultured splenocytes and B cells were measured by a mouse IL-10 enzyme-linked immunosorbent assay (ELISA) Max Standard kit (BioLegend, San Diego, CA, USA) using 1:10 assay diluent in PBST.

    Techniques: Isolation, Knock-Out, Immunocytochemistry, Staining, Enzyme-linked Immunosorbent Assay